mouse antibody subtype igg1  (MBL Life science)

Journal: The Journal of Cell Biology

Article Title: A global chromatin compaction pathway that represses germline gene expression during starvation

doi: 10.1083/jcb.202009197

Figure Lengend Snippet: Widespread H3K9 methylation is observed as chromatin compaction starts in C. elegans PGCs. (A) C. elegans embryos at different embryonic stages were fixed and stained for P-granules (green), H3K9me3 (red), and DNA (blue). Representative whole-embryo images are shown. (B) Same as A except only Z2/Z3 (germ) or a neighboring somatic cell (soma) is shown. The developmental stage of the embryo from which the image was taken is indicated ( n = 20). See for a summary of signal intensities. Scale bar represents a length of 2 µm.

Article Snippet: H3K9me2: Mouse antibody (subtype: IgG1) from Molecular and Biological Laboratories (MABI0317; MBL) was used at 1:1,000.

Techniques: Methylation, Staining

Journal: The Journal of Cell Biology

Article Title: A global chromatin compaction pathway that represses germline gene expression during starvation

doi: 10.1083/jcb.202009197

Figure Lengend Snippet: A TOP-2/condensin II–dependent increase in heterochromatin marks coincides with chromatin compaction in Z2/Z3. (A) Wild-type starved L1s and starved L1s that were defective for methyltransferases (mutants for met-2 , set-25 , set-32, and F1s from animals treated with met-2/set-25 double RNAi) were fixed and stained for P-granules (white), H3K9me2 (green), H3K9me3 (red), and DNA (blue). ( n = 40). See for a summary of signal intensities. (B) L1s were either starved or fed for a varying amount of time. Samples were then fixed and stained for P-granules (white), H3K9me3 (red), H3K9me2 (green), and DNA (blue). Representative images are shown. ( n = 40). See for a summary of signal intensities. (C) Starved L1s, born from strain N2 treated with either control RNAi or capg-2 RNAi, were fixed and stained for P-granules (white), H3K9me2 (green), H3K9me3 (red), and DNA (blue). Representative images are shown ( n = 40). See for a summary of signal intensities. (D) Starved L1s, born from strain N2 treated with either control or top-2 RNAi, were fixed and stained for H3K9me3 (red) and DNA (blue). Representative images are shown ( n = 40). See for a summary of signal intensities. (E) Worms that express HPL-2::mKate were optionally treated with control and capg-2 RNAi. Live embryos were extracted and were imaged for HPL-2 signal. The white star identifies Z2/Z3. Representative images are shown ( n = 20). (F) Quantification of HPL-2::mKate signal from the images taken in E. Error bars represent one standard deviation. Scale bar represents a length of 2 µm.

Article Snippet: H3K9me2: Mouse antibody (subtype: IgG1) from Molecular and Biological Laboratories (MABI0317; MBL) was used at 1:1,000.

Techniques: Staining, Control, Standard Deviation

Journal: The Journal of Cell Biology

Article Title: A global chromatin compaction pathway that represses germline gene expression during starvation

doi: 10.1083/jcb.202009197

Figure Lengend Snippet: Both H3K9 methyltransferases, MET-2 and SET-25, are needed for chromatin compaction in starved L1s. New and starved L1s born from strain WMM1 treated with either control RNAi, met-2 RNAi, or set-25 RNAi or met-2/set-25 double RNAi were used. Z2/Z3 from the L1s were imaged for chromatin compaction. Representative images are shown. Compaction was also quantified and shown below images. Error bars represent one standard deviation ( n = 20). Scale bar represents a length of 2 µm.

Article Snippet: H3K9me2: Mouse antibody (subtype: IgG1) from Molecular and Biological Laboratories (MABI0317; MBL) was used at 1:1,000.

Techniques: Control, Standard Deviation

Journal: International Journal of Molecular Sciences

Article Title: Berberine Delays Onset of Collagen-Induced Arthritis through T Cell Suppression

doi: 10.3390/ijms22073522

Figure Lengend Snippet: The effect of berberine on circulating anti-bovine type II collagen (CII) IgG in the CIA model. ( A ) Anti-CII IgG1, IgG2a, and total IgG at day 28 among all mice (arthritic and non-arthritic) within BBR, PBS (vehicle control), CIA (no treatment control), and non-CIA control animals ( n = 10 per group). ( B ) Anti-CII IgG levels at day 28 compared among arthritic mice only (BBR n = 5; PBS n = 9; CIA n = 9). Statistical comparisons made with the Kruskal–Wallis test with Dunn’s multiple comparisons. ( C ) Anti-CII IgG levels at day 28 compared among BBR-treated mice who developed arthritis (“arthritic”) vs. those that did not (“non-arthritic”). Statistical comparisons made with the Mann–Whitney U test. For all statistical tests in A–C, * p < 0.05, ** p < 0.01, **** p < 0.0001.

Article Snippet: Serum concentrations of anti-collagen type II (anti-CII) total IgG (catalog # 1012T), anti-CII IgG1 (catalog # 20321T), and anti-CII IgG2a (catalog # 20322T) were measured by ELISA (Chondrex, Redmond, WA, USA) according to the manufacturer’s instructions [ , ].

Techniques: MANN-WHITNEY

Journal: International Journal of Molecular Sciences

Article Title: Targeting of Phospholipase D1 Ameliorates Collagen-Induced Arthritis via Modulation of Treg and Th17 Cell Imbalance and Suppression of Osteoclastogenesis

doi: 10.3390/ijms21093230

Figure Lengend Snippet: PLD1 deficiency suppresses the collagen type II-specific humoral response and the production of proinflammatory cytokines in collagen-induced arthritis (CIA) mice. ( A ) The amount of anti-collagen total IgG, IgG1, and IgG2a antibodies was measured by ELISA in the serum from the indicated mice at day 42. ( B ) Measurement of proinflammatory cytokines in the serum of the indicated mice at day 42 as analyzed by ELISA. n = 15 per group. * p < 0.05, N.S., non-significant. Results are shown as mean ± standard error of the mean (SEM).

Article Snippet: The amount of murine CII-specific autoantibodies in the serum was measured using the Mouse Anti-Type II Collagen IgG Subtype Antibody Assay Kit (Chondrex), according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: International Journal of Molecular Sciences

Article Title: Targeting of Phospholipase D1 Ameliorates Collagen-Induced Arthritis via Modulation of Treg and Th17 Cell Imbalance and Suppression of Osteoclastogenesis

doi: 10.3390/ijms21093230

Figure Lengend Snippet: The PLD1 inhibitor reduces the production of the anti-CII IgG2a autoantibody and proinflammatory cytokines in collagen-induced arthritis (CIA) mice. ( A ) The amount of anti-collagen IgG1 and IgG2a antibodies was measured by ELISA in the serum of the indicated mice at day 45. ( B ) The amount of proinflammatory cytokines was measured by ELISA in the serum of the indicated mice at day 45. n = 15 per group. * p < 0.05. Results are shown as mean ± standard error of the mean (SEM).

Article Snippet: The amount of murine CII-specific autoantibodies in the serum was measured using the Mouse Anti-Type II Collagen IgG Subtype Antibody Assay Kit (Chondrex), according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: International Journal of Molecular Medicine

Article Title: Humulus japonicus extract ameliorates collagen-induced arthritis in mice through regulation of overall articular inflammation

doi: 10.3892/ijmm.2019.4417

Figure Lengend Snippet: Effect of Humulus japonicas (HJ) on the production of anti-type II collagen antibody in plasma. Levels of (A) anti-type II collagen total IgG and its subtypes (B) IgG1, and (C) IgG2a were measured by ELISA in plasma obtained on day 35 from each mice group. Gene expression levels of (D) Il-12rβ2 , (E) Ccr2 , (F) Ccr5 , (G) Ccr3 , (H) Ccr4 , (I) IL-2 and (J) IL-13 were analyzed by RT-qPCR in the paw of CIA mice on day 35 following the first immunization. The vehicle group was set to a value of 1, and average fold-change is shown. Grouped quantitative data are presented as means ± SEM (vehicle group; n=8, HJ group; n=6). Significance was measured using two-tailed Student's t-test. * P<0.05, ** P<0.01, *** P<0.001.

Article Snippet: Plasma samples were collected at the end of the experiment (day 35) for the determination of IgG, IgG1, and IgG2a antibody levels with three commercially available test kits, mouse anti-mouse Type II collagen IgG TMB (2036T, Chondrex), IgG1 TMB (20361T, Chondrex), and IgG2a (20362T, Chondrex) antibody subtype assay kit TMB, according to the manufacturer's instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR, Two Tailed Test

Journal: PLoS ONE

Article Title: Upregulation of sphingosine-1-phosphate receptor 3 on fibroblast-like synoviocytes is associated with the development of collagen-induced arthritis via increased interleukin-6 production

doi: 10.1371/journal.pone.0218090

Figure Lengend Snippet: (A) Development of collagen-induced arthritis in WT and S1P 3 knockout (S1P 3 -KO) mice. Arthritis was evaluated three times per week during Days 21–42. Disease severity was measured by assessing the clinical score of all four paws, in which the maximum possible score for an individual mouse was 16. Statistical analysis was performed at Days 36, 38, 40, and 42. Results are presented as the mean ± SEM (n = 20 mice per group) and were analyzed by t -test with Bonferroni’s correction. * P < 0.05 and ** P < 0.01 (WT vs. KO mice). (B) Synovial inflammation, bone erosion, and cartilage destruction were examined histologically on H&E-stained sections of ankle joints and assigned scores of 1–4. Results are presented as box-and-whisker plots (n = 20 mice per group) and were analyzed by Mann-Whitney U-test. The middle hash, the box, and the whiskers represent the median, interquartile range (IQR), and 10/90 percentile values respectively. * P < 0.05, NS = not significant. (C) Representative images of H&E-stained ankle joint sections are shown at a magnification of 40×. Left panel: WT specimen assigned a score of 3 for all parameters. Right panel: KO specimen assigned a score of 1 for inflammation and 0 for the other parameters. (D) Anti-CII IgG 1 and IgG 2 antibodies in serum from CIA mice were measured in an ELISA. Results are presented as box-and-whisker plots (n = 10 mice per group) and were analyzed by Mann-Whitney U-test. The middle hash, the box, and the whiskers represent the median, IQR, and minimum/maximum values respectively. (E) Expression of TNFα and IL-6 in arthritic synovium was analyzed by real-time RT-PCR. Results are presented as the mean ± SEM (n = 6 mice per group) and were analyzed by t -test. * P < 0.05, NS = not significant.

Article Snippet: Levels of anti-CII IgG 1 and IgG 2 were measured using a Mouse Anti-type II Collagen IgG Subtype Antibody ELISA Kit (Chondrex), according to the manufacturer’s instructions.

Techniques: Knock-Out, Staining, Whisker Assay, MANN-WHITNEY, Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR